Part:BBa_K5186013

shRNA3(5-HTR1)

To combat mosquito populations, shRNAs (5-HTR1), variants of short hairpin RNAs, are engineered to target mosquitoes' 5-HTR1 gene, which encodes serotonin(5-HT) receptors crucial for immune and nervous systems, utilizing RNAi technique. When shRNA3(5-HTR1) in the freeze dried inactivated yeast cells are digested by mosquitoes, they will be processed into small interfering RNAs (siRNAs), and further specifically target mRNA(5-HTR1) starting at 1408 nt to achieve degradation. This targeted interference results in neural misfunctions in mosquitoes, eventually causing their deaths without any harm to other non-target organisms. These shRNAs(5-HTR1) (BBa_K5186011, BBa_K5186012, BBa_K51860013) and other shRNAs targeting mosquitoes' vital survival genes (BBa_K5186014, BBa_K5186015, BBa_K5186016, BBa_K5186017) contribute to mosquitoes control solutions and thus make up a part collection. This collection serves as a valuable resource for the iGEM community and researchers, offering a safe, efficient, and environmentally friendly approach to mosquito control.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]