Device

Part:BBa_K5152009

Designed by: Tsz Yan Lai   Group: iGEM24_HongKong-JSS   (2024-09-24)
Revision as of 09:09, 24 September 2024 by Registry (Talk | contribs)

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pZnt zinc sensing chromoprotein reporter device

This device contains the zinc-inducible promoter pZnt, natively found in E. coli, to drive the expression of the chromoprotein eforRed as the reporter signal. As a result, we did not co-express any regulatory proteins in our design. However, our results showed significant leaky expression even in the absence of zinc, indicating that the promoter's repression needs to be strengthened in future designs to improve specificity and reduce background expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None