Part:BBa_K1119005

CMV promoter

Constitutive promoter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Information provided by CUHK-HongKong-SBS 2024

The cytomegalovirus (CMV) promoter is a complex transcription factor regulatory element (TFRE) for various commonly expressed TF (1). The promoter activity is regulated by a system-specific combination of interactions with its respective TF, examples of its activity being activated by members of the MAP/ERK kinase kinase 1 (Mekk1) -JNK-AP-1 pathway have been proven, and being repressed by p53 when it attaches and interacts with the TATA binding protein and/or basal transcriptional machinery of the CMV promoter (2). Therefore, its activity is deemed as cell-type dependent. Functions of the CMV promoter include but are not limited to involvement in DNA manipulation of embryonic stem cells (3).

References

1. Johari, Y. B., Scarrott, J. M., Pohle, T. H., Liu, P., Mayer, A., Brown, A. J., & James, D. C. (2022). Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnology Journal, 17(8), 2200062. https://doi.org/10.1002/biot.202200062

2. Rodova, M., Jayini, R., Singasani, R., Chipps, E., & Islam, M. R. (2013). CMV promoter is repressed by p53 and activated by JNK pathway. Plasmid, 69(3), 223–230. https://doi.org/10.1016/j.plasmid.2013.01.004

3. Barrow, K. M., Perez-Campo, F. M., & Ward, C. M. (2006). Use of the cytomegalovirus promoter for transient and stable transgene expression in mouse embryonic stem cells. Methods in Molecular Biology (Clifton, N.J.), 329, 283–294. https://doi.org/10.1385/1-59745-037-5:283