Device

Part:BBa_K5152006

Designed by: Tian Xin Ho   Group: iGEM24_HongKong-JSS   (2024-09-24)
Revision as of 09:04, 24 September 2024 by Registry (Talk | contribs)

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MerR-pCadA cadmium sensing chromoprotein reporter device

This device includes the cadmium-inducible pCadA promoter and co-expresses its repressor MerR, which has been specifically modified to bind cadmium. In the presence of cadmium, the pCadA promoter activates the expression of the downstream chromoprotein amilCP, serving as the reporter signal. This device is specifically designed to detect the presence of cadmium in the environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 350
    Illegal AgeI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None