Composite

Part:BBa_K243012

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 16:47, 21 October 2009 by Timo (Talk | contribs)

Strep-DigA-Middle Linker-Fok_i

This part unites some of our basic parts. The combination of a StrepTag, a Anticalin-Tag and the linked protein domain Fok_i, makes it possible to purifier and detect the function of the protein. The DigoxigeninA tag allows the coupling to an digoxigenin linked oligonucleotide.

Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to built to a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an Digoxigenin labeled oligonucleotide. The middle linker mades a distance from 24bp between the DigA and the linked Fok_i protein domain. The StrepTag serve as purification tag for Streptavidin column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None