Part:BBa_K5186003

sRNA3(IspH)

sRNA(IspH)s are synthetic small regulatory RNAs (sRNAs) designed to specifically down-regulate the expression of the IspH gene in E. coli, thereby enhancing HMBPP production for mosquito attraction. Each sRNA(IspH) is composed of a target-binding sequence that is complementary to the IspH mRNA, enabling hybridization, and a SgrS scaffold that facilitates the recruitment of the Hfq protein in E. coli. This interaction promotes the sRNAs' binding to ispH mRNA and expedites its degradation by RNase E. (Seung Min Yoo et al.) It has been revealed that the mutation in the SgrS scaffold can enhance the efficiency of sRNA-mediated gene repression (Minho Noh et al.). This year, we have successfully engineered 4 variants of sRNA(IspH)s in E. coli, each with a specialized scaffold. We expect to select the best one for controlling the down-regulation of IspH from them. sRNA3(IspH) features a CUUU 6-nts stem with the SgrS-S scaffold, while sRNA2(IspH) with a SgrS-S 6-nts stem scaffold exhibits the most excellent downregulation effect without affecting bacterial growth (BBa_K5186002).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]