Composite

Part:BBa_K5186005

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-24)
Revision as of 07:34, 24 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PTac-riboJ-DXS-IspG-IspDF-B0015-PTac-sRNA2(IspH)-B0015

PTac-sRNA2(IspH)-B0015 is an expression cassette in E. coli expressing sRNA2(IspH) (BBa_K5186002), which exhibits the most excellent downregulation effect on IspH gene without affecting bacterial growth in E. coli. With this expression cassette, sRNA2(IspH) can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli. It can be used to enhance HMBPP production for mosquito attraction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4325
    Illegal BglII site found at 5541
    Illegal BamHI site found at 3653
    Illegal BamHI site found at 4869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2102


[edit]
Categories
Parameters
None