Translational_Unit

Part:BBa_K216017:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-21)
Revision as of 16:37, 21 October 2009 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


luxCDE genes of Xenorhabdus luminescens (aldehyde biosynthesis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1332


Design Notes

The normal operon structure is luxCDABE. This part is derived from an earlier (non-BioBrick) project, in which the luxCD genes were fused to luxE so that the aldehyde biosynthesis machinery could be induced separately from the luciferase. An XbaI site must be removed from within luxD.


Source

Amplified from genomic DNA of Xenorhabdus luminescens Hb (NCIMB 12670).

References