Generator
Part:BBa_K210005:Design
Designed by: Makoto Kashima Group: iGEM09_Kyoto (2009-10-13)
LacI generator yeast-optimized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1569
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This CDS has SV40 nuclear localization sequence. But, due to frameshift, this does not successfully translate.
Source
BBa_K165037 BBa_C0012 BBa_K165018
References
http://en.wikipedia.org/wiki/Kozak_consensus_sequence