Coding

Part:BBa_K5317016:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)
Revision as of 16:59, 23 September 2024 by Vanessa09 (Talk | contribs) (→‎References)

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ATF2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1031


Design Notes

This sequence was codon optimised for human cell lines.


Source

We obtained this sequence from S. aureus.