Part:BBa_K5143005

Description

pUC57 backbone we used is composed of the Ampicillin Resistance gene (to select transformant colonies), the replication origin ColE1 (enables the plasmid replication in E. coli in high-copy-number), the URA3 selection marker (enables the selection of recombinant yeast) and the Ura3' and Ura5'homologies region (enables the recombination of gene of interest in the yeast).

Construction

This part has been synthesized in order to have the URA3 gene and the Ura3'/Ura5' homologies region.

References

mettre des réf

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 3440
  Illegal EcoRI site found at 3967
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3440
  Illegal EcoRI site found at 3967
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3973
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3440
  Illegal EcoRI site found at 3967
  Illegal XhoI site found at 1605
  Illegal XhoI site found at 3446
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal suffix found at 2
  Illegal EcoRI site found at 3440
  Illegal EcoRI site found at 3967
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3440
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 714