Part:BBa_K5283019
P9, an 84 kDa thermogenesis-inducing protein secreted by Akkermansia muciniphila, enhances GLP-1 sec
The P9 protein gene from Akkermansia muciniphila is a bioengineered component with significant implications for the treatment of metabolic disorders such as diabetes and obesity. This gene encodes for an 84 kDa protein that has been shown to stimulate the secretion of glucagon-like peptide-1 (GLP- 1), a key regulator of energy metabolism and glucose homeostasis. The therapeutic potential of P9 lies in its ability to interact with intercellular adhesion molecule 2 (ICAM-2), which is crucial for its metabolic effects.
In practical terms, the P9 protein can be heterologously expressed in other organisms, such as Lactococcus lactis, to produce and secrete the protein. This approach has been successfully demonstrated, where the engineered L. lactis not only secreted P9 but also stimulated GLP-1 production in NCI-H716 cells, a model for intestinal L cells. The relative expression levels of GLP-1 biosynthesis genes GCG and PCSK1 were upregulated, indicating the potential of this engineered strain for therapeutic use.
For users interested in incorporating the P9 protein gene into their projects, it can be used as a genetic component to develop probiotics or other biotherapeutics aimed at improving glucose metabolism and treating metabolic diseases. The gene can be codon-optimized for the host organism and expressed under controlled conditions to ensure optimal protein production and secretion.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 43
Illegal PstI site found at 1372
Illegal PstI site found at 1744 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 43
Illegal PstI site found at 1372
Illegal PstI site found at 1744 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 123
Illegal BamHI site found at 483
Illegal BamHI site found at 1977 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 43
Illegal PstI site found at 1372
Illegal PstI site found at 1744 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 43
Illegal PstI site found at 1372
Illegal PstI site found at 1744 - 1000COMPATIBLE WITH RFC[1000]
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