Reporter

Part:BBa_K191004:Design

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-11)
Revision as of 16:06, 21 October 2009 by Nltt1987 (Talk | contribs) (→‎Design Notes)

TRP promoter - RBS - RFP - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
    Illegal AgeI site found at 666
    Illegal AgeI site found at 778
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Trp promoter was added to plasmid containing part BBa_I13507 from iGEM kit using [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow Klenow protocol].
  • Trp promoter as PCR products in Klenow protocol was cut with E and S to be ligated into plasmid with RFP gene (BBa_I13507), which was also cut with E and S.

Source

BBa_K191007, BBa_I13507

References