Coding

Part:BBa_K5301013

Designed by: xuan zhang   Group: iGEM24_BNU-China   (2024-09-23)
Revision as of 07:28, 23 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


spNW15

SpNW15 is the truncated form of spNW30. spNW15 could construct nanodiscs with diameter of 15nm, and is circularized by SpyTag-Spycatcher.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal NheI site found at 64
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BglII site found at 552
    Illegal BglII site found at 924
    Illegal BamHI site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal AgeI site found at 1027
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None