Composite

Part:BBa_K5208014

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-BioX   (2024-09-23)
Revision as of 06:39, 23 September 2024 by Registry (Talk | contribs)

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Pgrac-SPamyQ-AhlD-Term

This is an expression cassette consisting of a strong inducible promoter Pgrac, a secretion signal peptide SPamyQ, the AHL lactonase AhlD, and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 827
    Illegal BamHI site found at 941
    Illegal XhoI site found at 526
    Illegal XhoI site found at 640
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 523
    Illegal BsaI.rc site found at 247


[edit]
Categories
Parameters
None