Composite

Part:BBa_K5208013

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-BioX   (2024-09-23)
Revision as of 06:08, 23 September 2024 by Registry (Talk | contribs)

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Pgrac-SPamyQ-AiiM-Term

This is an expression cassette consisting of a strong inducible promoter Pgrac, a secretion signal peptide SPamyQ, and the AHL lactonase AiiM, and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
    Illegal BamHI site found at 788
    Illegal XhoI site found at 739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 695
    Illegal BsaI site found at 823
    Illegal SapI site found at 510


[edit]
Categories
Parameters
None