Coding

Part:BBa_K5392000

Designed by: ZHENQIANG SUN   Group: iGEM24_LIUAN-Nanjing   (2024-09-23)
Revision as of 03:50, 23 September 2024 by Registry (Talk | contribs)

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ZaTdT

ZaTdT is a polymerase whose predominant activity is to indiscriminately add deoxynucleotide triphosphates (dNTPs) to the 3 end of single stranded DNA.We want to use a ZaTdT and perform site directed mutagenesis of its DNA sequence through computer simulation to obtain ZaTdT mutants that can catalyze modified nucleotides. Protein expression will be performed in Escherichia coli BL21.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None