Coding

Part:BBa_K5286001:Design

Designed by: Mara Valverde Rascón   Group: iGEM24_TUM-Straubing   (2024-09-22)
Revision as of 21:13, 22 September 2024 by Maravalverde (Talk | contribs) (→‎Source)


Cytosolic-abundant heat soluble protein 94063 (CAHS94063)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNA retrotrasncription and it has already been codon optimized for expression in E. coli.



Source

The part was obtained via RNAseq of Hypsibius dujardini under desiccation conditions by Boothby et al.(2017)

References

Boothby, T. C., Tapia, H., Brozena, A. H., Piszkiewicz, S., Smith, A. E., Giovannini, I., Rebecchi, L., Pielak, G. J., Koshland, D., & Goldstein, B. (2017). Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation. Molecular Cell, 65(6), 975. https://doi.org/10.1016/j.molcel.2017.02.018