Part:BBa_K5317021

CMV-ATF2-mRuby2

ATF2 belongs to the ATF/CREB family and regulates genes involved in cell growth, stress responses and apoptosis. With the addition of the ATF2 gene, this plasmid enables the study of transcriptional regulation of ATF2 (Kirsch et al., 2020) and its phosphorylation by PknB, making it important for research into signaling pathways related to cell stress and survival. It also could play a role in modulating bacterial virulence by altering key regulatory proteins, affecting their role in infection or antibiotic resistance. (Zhang et al., 2020) while mRuby2 provides a fluorescent marker for visualization. In our cell-based β-lactam ring-containing antibiotics sensor, ATF2 serves as a translator of changes in PknB activity at the level of gene regulation, in particular the activity of the ATF2-3xCre2xAP1 promoter.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1398
  Illegal EcoRI site found at 1660
  Illegal XbaI site found at 1373
  Illegal XbaI site found at 1701
  Illegal PstI site found at 2108
  Illegal PstI site found at 2557
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1398
  Illegal EcoRI site found at 1660
  Illegal PstI site found at 2108
  Illegal PstI site found at 2557
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1398
  Illegal EcoRI site found at 1660
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1398
  Illegal EcoRI site found at 1660
  Illegal XbaI site found at 1373
  Illegal XbaI site found at 1701
  Illegal PstI site found at 2108
  Illegal PstI site found at 2557
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1398
  Illegal EcoRI site found at 1660
  Illegal XbaI site found at 1373
  Illegal XbaI site found at 1701
  Illegal PstI site found at 2108
  Illegal PstI site found at 2557
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 670
  Illegal SapI.rc site found at 2408