Coding

Part:BBa_K5317016:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)
Revision as of 13:51, 22 September 2024 by Vanessa09 (Talk | contribs) (→‎References)


ATF2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 283
    Illegal XbaI site found at 324
    Illegal PstI site found at 731
    Illegal PstI site found at 1180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1031


Design Notes

This sequence was codon optimised for human cell lines.


Source

We obtained this sequence from S. aureus

References

Kirsch, K., Zeke, A., Tőke, O., Sok, P., Sethi, A., Sebő, A., Kumar, G. S., Egri, P., Póti, Á. L., Gooley, P., Peti, W., Bento, I., Alexa, A., & Reményi, A. (2020). Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38. Nature Communications, 11(1), 5769. https://doi.org/10.1038/s41467-020-19582-3


Zhang, C., Zhang, R., Dai, X., Cao, X., Wang, K., Huang, X., & Ren, Q. (2020). Activating transcription factor 2 (ATF2) negatively regulates the expression of antimicrobial peptide genes through tumor necrosis factor (TNF) in Macrobrachium nipponense. Fish & Shellfish Immunology, 107, 26–35. https://doi.org/10.1016/j.fsi.2020.09.043