Coding

Part:BBa_K5321014

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 13:36, 22 September 2024 by Registry (Talk | contribs)

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SA_nPPVp

Given the challenges associated with the expression of proteins incorporating unnatural amino acids, we are exploring natural bioaffinity systems that can be constructed using simply fused proteins. We have selected streptavidin-biotin interactions for this purpose. Streptavidin (SA) is a tetrameric protein derived from the bacterium Streptomyces Avidini, which exhibits extraordinary affinity for biotin. Streptavidin (SA) can be easily fused with the split protease, and biotinylated aptamers can be readily obtained.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 853
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 853
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 853
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 853
    Illegal NgoMIV site found at 508
    Illegal AgeI site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None