Part:BBa_K5246008

CB2/CB2A HfsH Deacetylase

Usage and Biology

HfsH encodes a cytoplasmic deacetylase of 257 amino acids long. Deacetylase belongs to carbohydrate esterase family 4, catalyze the hydrolysis of N-linked acetyl groups from GlcNAc residues. C.Crescentus HfsH mutants were completely devoid of holdfast material Another C. crescentus CB15 mutant, ΔhfsH (YB2198), was used to study the role of deacethylation in adhesion efficiency. Indeed, this mutant is lacking the gene hfsH, encoding a deacetylase that affects both cohesive and adhesive properties of the holdfast.30 C. crescentus ΔhfsH produces smaller holdfasts compared to the wild-type and the ΔhfaB strains. These fully acetylated holdfasts are not anchored properly to the cell envelope and are shed in the medium. [2] The main strain used in this study was Caulobacter crescentus CB15 ΔhfaB (YB4251),29 a mutant strain from C. crescentus CB15 wild-type (YB135). This mutant has a clean deletion of the hfaB gene and therefore does not synthesize HfaB, one of the holdfast anchor proteins. This strain still produces a holdfast, but is unable to anchor it to the cell envelope. As a consequence, the newly synthesized holdfast is shed in the culture medium and on surfaces. [3]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 10
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 10
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 10
  Illegal NgoMIV site found at 68
  Illegal NgoMIV site found at 92
  Illegal NgoMIV site found at 96
  Illegal NgoMIV site found at 169
  Illegal NgoMIV site found at 295
  Illegal NgoMIV site found at 456
  
• 1000
  COMPATIBLE WITH RFC[1000]