Part:BBa_K5226049
TcI42-T64
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to build thermosensitive bio-switch for Halomonas TD.We successfully built one using ci857 regulatory protein. And for better performance and broader range of usage, we mutated CI857 and its mutant TCI-42 to see whether we could get one or several well-performed mutant with lower leakage and higher fold change.
Usage and Biology
T64 is a mutant of TCI-42,a widely used mutant of cI from bacteriophage λ as a thermogenetic tool. At 30℃ ,The cI857 dimer repressor binds in a cooperative form, by protein-protein interactions of their C-terminal domains that exhibits strong repression on R promoter. At 37℃, the dimer dissociates, So the R promoter works.After our verification by experiment, C27 has lower leakage and higher fold change than CI857.
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