Part:BBa_K5246005
CB2/CB2A HfsE Integral membrane glycosyltransferases
Encodes a protein of 512 amino acids, integral membrane glycosyltransferases from the polyisoprenylphosphate hexose-1-phosphate transferase (PHPT) family , transferrs hexose-1-phosphate residues from UDP-hexoses to the lipid carrier molecule undecaprenol phosphate in the inner membrane. In C.Crescentus catalyzes the first step in polysaccharide biosynthesis by transferring glucose-1-phosphate residues from UDP-glucoses to the lipid carrier molecule undecaprenol phosphate in the inner membrane Studies using hfsE mutants showed minimal changes in holdfast synthesis and bacterial adhesion. Through further genome analysis two paralogs, pssY and pssZ, were identified as potential substitutes for the hfsE protein in holdfast synthesis. However, double combination mutants did not have a substantial decrease of neither surface adherence nor inability to synthesize holdfast, but had a slight misplacement at the tip of the stalk. While hfsE, pssY and pssZ triple deletion mutant, was unable to produce holdfast or adhere to surfaces. [2]"
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 530
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 669
Illegal NgoMIV site found at 1300
Illegal AgeI site found at 999 - 1000COMPATIBLE WITH RFC[1000]
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