Device

Part:BBa_K177024

Designed by: Frank Fijalkowski   Group: iGEM09_Warsaw   (2009-07-10)
Revision as of 15:34, 21 October 2009 by Messer (Talk | contribs) (→‎Usage and Biology)

LacI detector device


This device was constructed as a test to determine which of the E. coli strains available were truly lacI-.


Usage and Biology

When tested strains transformed only with this brick are incubated without IPTG and green fluorescence is observed then the bacteria are lacI-.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 873


[edit]
Categories
Parameters
n/aGFP under LacI promotor