Coding

Part:BBa_K5226045

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-22)
Revision as of 12:04, 22 September 2024 by Admin (Talk | contribs)

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fmo

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 484
    Illegal AgeI site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.

Usage and Biology

MaFMO is a kind of flavin-containing monooxygenase. The protein sequence is from Methylophaga aminisulfidivorans. This protein catalyzes the conversion 6-Br-indole into 6,6'-dibromoindigo (6BrIG, also known as tyrian purple).


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