Composite
His-FluA-S

Part:BBa_K243010:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 15:32, 21 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

His-FluA-Split Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning steps were planed theoretically before we started the work in the wet lab. The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain of our universal endonuclease. The parts are fused with RFC 25.
Commented GenBank file

Source

Combined the parts by serial cloning steps.

References