Primer

Part:BBa_K5269001

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-22)
Revision as of 06:57, 22 September 2024 by Registry (Talk | contribs)

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AAAAA

This reverse primer is used with the pBl_InF fwd1 primer(BBa_K5269002) in the pBluescript plasmid for PCR and In-Fusion for Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 15
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None