Coding

Part:BBa_K5321012

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 05:45, 22 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


cPPVp_mut

To construct a molecular machine featuring a highly efficient sensor, such as an aptamer, and an upstream signal transporter, like a split protease, we have devised crosslinking via click chemistry, specifically DBCO-p-AzF click reaction, to do this task. We introduced UAG stop codon site-directed mutations into the cPPVp genes. This modification allows the pEvol-pAzFRS.2.t1 plasmids to incorporate p-AzF through an orthogonal tRNA synthetase/tRNA pair.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None