Device

Part:BBa_K177025

Designed by: Frank Fijalkowski   Group: iGEM09_Warsaw   (2009-07-10)
Revision as of 15:25, 21 October 2009 by Messer (Talk | contribs)

GFP under araC promotor


This device was constructed as a test to determine which of the E. coli strains available were truly araC-.


Usage and Biology

When tested strains transformed only with this brick are incubated with arabinose and no green fluorescence is observer then the bacteria are araC-.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1013
    Illegal SapI site found at 107


[edit]
Categories
Parameters
n/aGFP under araC promotor