Coding

Part:BBa_K5321010

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 02:46, 22 September 2024 by Registry (Talk | contribs)

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GFP_truncated_linker(TEV)_YFP_truncated

We used GFP_truncated_linker(TEV)_YFP_truncated as a cleavage substrate for Plum pox virus protease (TEVp), which is formed by a TEVp cleavage site (ENLYFQS) with truncated Green fluorescent protein (GFP) and the truncated Yellow fluorescent protein (YFP) at both ends, and there is a linker between each of the two, consisting of G and S. When it is cleaved by TEVp, two bands of different sizes can be observed by SDS-PAGE electrophoresis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
    Illegal AgeI site found at 159
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None