Part:BBa_K257002

ClyA_Fusion_C-Term

ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.

PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.

• ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
• We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
• ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
• Cly A confers vesicle binding to and invasion of host cells.
• ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 85
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]