Part:BBa_K5332001
Reactive oxygen species promoter
ROS, or reactive oxygen molecules, can accumulate abnormally in certain environments and affect the occurrence of diseases such as IBD. In order to design a promoter that can start expression in an environment with high reactive oxygen concentrations, we selected the promoter of the KatG gene that can respond to reactive oxygen stress. Referring to the relevant literature on the experimental analysis of reactive oxygen stress response of katG gene alone, it can be seen that the expression of katG increased gradually at first, and then declined after simulation is saturated. The initial upregulation is the result of increased H2 O2 output by aerobic metabolism under favorable conditions. The activity of peroxidase, a gene expression product, also increased first and then remained stable. This mechanism can reduce the extra energy expenditure of cells. Therefore, the katG promoter is suitable for designing elements to regulate the expression of target genes in a spatiotemporal specific manner.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 182
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 182
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 182
Illegal BglII site found at 253 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 182
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 182
- 1000COMPATIBLE WITH RFC[1000]
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