Device

Part:BBa_K195623

Designed by: Yun-Hsiang Chang   Group: iGEM09_NYMU-Taipei   (2009-10-18)
Revision as of 15:16, 21 October 2009 by Benjamin (Talk | contribs)

CII/PenI Damped Oscillator With GFP Reporter (II)

This device is produces damped oscillations between PenI and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces PenI (C0074) and GFP. The PenI then represses the pPenI promoter (R0074) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1927


[edit]
Categories
Parameters
None