Coding

Part:BBa_K5321009

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-21)
Revision as of 13:45, 21 September 2024 by Registry (Talk | contribs)

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GFP_truncated_linker(PPV)_YFP_truncated

We used GFP_truncated_linker(TEV)_YFP_truncated as a cleavage substrate for Tobacco Etch Virus protease (TEVp), which is formed by a TEVp cleavage site (ENLYFQS) with truncated Green fluorescent It is formed by the TEVp cleavage site (ENLYFQS), which is connected to the truncated Green fluorescent protein (GFP) and the truncated Yellow fluorescent protein (YFP) at both ends, and there is a linker between each of the two, consisting of G and S. When it is cleaved by TEVp, two bands of different sizes can be observed by SDS-PAGE electrophoresis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
    Illegal AgeI site found at 159
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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