Composite
Part:BBa_K5036032
Designed by: Emad hamdy Matter Group: iGEM24_AFCM-Egypt (2024-09-21)
dCas9(C)_NES-Syn-VEGFR-2 (VEGF-R2, N-TEV, NES, TCS (Q, L), HA, dCas9(C),mCherry
In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to a protein sequence containing TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS), and dCas9(N). When an injury occurs, VEGF levels rise outside the cell. This binds to VEGF-R2, activating the receptor and triggering the TEV protease to cleave the chain at the TCS site. This releases dCas9(N), which can then associate with a complementary dCas9 fragment (dCas9(C)) to form a fully functional dCas9 enzyme
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 982
Illegal BglII site found at 2341 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3976
Illegal NgoMIV site found at 4049
Illegal NgoMIV site found at 4534
Illegal NgoMIV site found at 5443 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1854
Illegal BsaI.rc site found at 664
Illegal BsaI.rc site found at 1442
Illegal BsaI.rc site found at 2731
Illegal SapI.rc site found at 3289
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Categories
Parameters
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