Coding

Part:BBa_K5387001

Designed by: iGEM24_Linkoping   Group: iGEM24_Linkoping   (2024-08-20)
Revision as of 10:39, 21 September 2024 by Feliciawedlund (Talk | contribs)

ALOXE3

Usage and Biology

ALOXE3 encodes for the enzyme eLOX3, which is very similar to 12-LOX in that it is coded by a gene located on the same chromosome and has the same function in the skin. However, the mechanism is slightly different. Instead of eLOX adding an oxygen molecule to the substrate to create fatty acid hydroperoxide, eLOX is involved in the hydroperoxide becoming a signaling molecule [1].

The gene codes for one of the enzymes used for iGEM24_Linkoping substitution therapy for Ichthyosis.

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
    Illegal BglII site found at 1541
    Illegal BglII site found at 1646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1920

References

[1]: ALOXE3 gene: MedlinePlus Genetics [Internet]. Available from: https://medlineplus.gov/genetics/gene/aloxe3/

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Categories
Parameters
None