Composite

Part:BBa_K5036029

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)
Revision as of 09:59, 21 September 2024 by Registry (Talk | contribs)

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dCas9(C)_NLS-Syn-VEGFR-1 (VEGF-R1, C-TEV, NLS, TCS(Q,L), HA,dCas9(C),VP64,GFP)

In our first receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R1, is attached to a protein sequence containing TEV protease, a nuclear localization signal (NES), a TEV cleavage site(TCS), and dCas9(C). When an injury occurs, VEGF levels rise outside the cell. This binds to VEGF-R1, activating the receptor and triggering the TEV protease to cleave the chain at the TCS site. This releases dCas9(C), which can then associate with a complementary dCas9 fragment (dCas9(N)) to form a fully functional dCas9 enzyme


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2173
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3973
    Illegal NgoMIV site found at 4046
    Illegal NgoMIV site found at 4531
    Illegal NgoMIV site found at 5440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6737
    Illegal SapI.rc site found at 3268


[edit]
Categories
Parameters
None