Translational_Unit

Part:BBa_K216008:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)
Revision as of 14:57, 21 October 2009 by Cfrench (Talk | contribs) (→‎References)

bacterial luciferase LuxAB of Xenorhabdus luminescens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


Design Notes

No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.


Source

Amplified from genomic DNA of Xenorhabdus luminescens strain Hw.

References

  • Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
  • Valkova, N., Szittner, R., and Meighen, E.A. 1999. Control of luminescence decay and flavin binding by the LuxA carboxy-terminal regions in chimeric bacterial luciferases. Biochemistry 38, 13820-13828.
  • Westerlund-Karlsson, A., Saviranta, P., and Karp, M. 2002. Generation of thermostable monomeric luciferases from Photorhabdus luminescens. Biochemical and Biophysical Research Communications 296, 1072-1076.
  • Mitchell, R.J., Ahn, J.M., and Gu, M.B. 2005. Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns. Journal of Microbiology and Biotechnology 15, 48-54.