Composite

Part:BBa_K5066007

Designed by: Abner Tseng and Megan Chiao   Group: iGEM24_GEMS-Taiwan   (2024-09-21)
Revision as of 05:56, 21 September 2024 by Abnert (Talk | contribs) (→‎Description)

Description

The modifications allow various functions to be possible for our DNA strain. The ribosome binding site(RBS) is a sequence of nucleotides before the start codon of an mRNA transcript and functions to recruit ribosomes for the translation of proteins. The histag,6X His, is a commonly used purification tag that contains 6 consecutive histidine residues. The His-tag can be stained with His-tag antibodies after translation, labelling the target recombinant protein. It is typically placed on either the N or C terminus of a protein. The strain is also has its stop codons removed in order to increase the production of toxins. P20 is a chaperone that aids in the production of BTI toxins.

Use in Biology

Cyt2ba is a toxin that binds to membrane receptors and increases membrane permeability. In Aedes aegypti, the toxin binds to the midgut of the mosquito larvae. Due to its crystalline structure, the toxin alters the cell membrane’s permeability which is crucial for cellular transport and activities, ultimately leading to the death of the larvae as a result of malnutrition and damage to the cell membrane. BmK produces a scorpion toxin that acts as a neurotoxin, and it is a type of toxin that is selectively active on lepidopterous and dipterous insects.Due to its high insect toxicity and its selectivity for only lepidopterous and dipterous insects, scorpion toxins can be applied as potential biological insecticides in pest control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 485
    Illegal EcoRI site found at 879
    Illegal EcoRI site found at 1192
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 485
    Illegal EcoRI site found at 879
    Illegal EcoRI site found at 1192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 485
    Illegal EcoRI site found at 879
    Illegal EcoRI site found at 1192
    Illegal BglII site found at 1640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 485
    Illegal EcoRI site found at 879
    Illegal EcoRI site found at 1192
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 485
    Illegal EcoRI site found at 879
    Illegal EcoRI site found at 1192
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None