Coding

Part:BBa_K5301001

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-21)
Revision as of 04:41, 21 September 2024 by Registry (Talk | contribs)

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SdyCatcher can achieve covalent binding of proteins through Tag-Catcher interaction.

SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 58
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 256


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Parameters
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