Coding
Part:BBa_K5396006
Designed by: Alex Johan Mendes Comodaro Group: iGEM24_CNPEM-BRAZIL (2024-09-17)
Revision as of 16:33, 20 September 2024 by Jocomodaro (Talk | contribs)
T7-Nt-BaCBM2-Cys
This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).
Usage and Biology
Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability.
Part Generation
Gibson Assembly
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 96
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 30
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 96
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 96
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |