Part:BBa_K5398613
Codon-Optimized EL222 Regulatory Element, improved from Part:BBa_K2332004
Our team has optimized the codon usage of the natural photosensitive DNA-binding protein EL222, originally from the marine bacterium Erythrobacter litoralis HTCC2594, for efficient expression in Escherichia coli. In the dark, EL222 remains in its inactive form, where the N-terminal LOV domain suppresses the DNA-binding activity of its C-terminal HTH domain. Upon exposure to blue light (450nm), the interaction between the LOV and HTH domains is released, allowing the protein to dimerize and bind to its specific DNA target regions. By optimizing the codon usage, we have significantly enhanced its expression in E. coli, providing a more efficient tool for light-controlled gene regulation studies.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 320
Illegal BamHI site found at 138 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 196
Illegal AgeI site found at 306 - 1000COMPATIBLE WITH RFC[1000]
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