Device

Part:BBa_K283023

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-11)
Revision as of 14:42, 21 October 2009 by NelsonChu (Talk | contribs)

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plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator before cheZ gene. We intend use this device to control the expression of cheZ and bactomotors' movement.This part does not pose any biological threat.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/device/uncategorized
//function/motility
Parameters
None