Composite

Part:BBa_K5396007

Designed by: Alex Johan Mendes Comodaro   Group: iGEM24_CNPEM-BRAZIL   (2024-09-17)
Revision as of 11:58, 20 September 2024 by Jocomodaro (Talk | contribs)


R0010-BaCBM2-Cys

This composite part codes for the BaCBM2 protein with an additional amino acid (cysteine)(BBa_K53960003). his enhancement allows it to be effectively utilized in our biosensor technology.

This part consists of the following basic parts: BBa_R0010, BBa_J435345, BBa_K53960003 and BBa_J428069.

Usage and Biology

This CBM2, or Carbohydrate-Binding Module 2, is a protein sourced from Bacillus anthracis. It belongs to a broader family of carbohydrate-binding modules that are crucial for the degradation of polysaccharides. These modules are important to break down complex carbohydrates, enabling microorganisms to convert them into usable energy sources. </p>

Recent study [ ] has shown that CBM2 has the ability to bind to certain types of plastics, especially those derived exhibiting similar structural features of polysaccharides. This binding ability is largely due to the protein's carbohydrate-binding properties, which facilitate interactions with specific functional groups found on plastic surfaces.

Part Generation

Golden Gate

Expression and purification

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None