Protein_Domain
AIDA-I

Part:BBa_K257018:Design

Designed by: Soufiane Boumahdi   Group: iGEM09_Paris   (2009-10-18)
Revision as of 14:28, 21 October 2009 by Sboumahdi (Talk | contribs) (→‎Design Notes)

AIDA-I autotransporter, linker + translocator domains


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1118
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane. AIDA Translocator domain has been designed using a fusion silver standard 23 prefix in order to be able to make a fusion protein with the N terminal extremity of AIDA translocator.

Source

AIDA has been synthesized in vitro.

References