Coding
FecI

Part:BBa_K257017:Design

Designed by: Soufiane Boumahdi   Group: iGEM09_Paris   (2009-10-18)
Revision as of 14:24, 21 October 2009 by Sboumahdi (Talk | contribs) (→‎Source)

Fec I, part of the signal transduction pathway FecARI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 338


Design Notes

Fec I needs to be expressed in association with Fec R behind the Pfec promoter in order to have a functional transduction pathway.


Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References