Coding

Part:BBa_K191008:Design

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-19)
Revision as of 14:19, 21 October 2009 by Nltt1987 (Talk | contribs) (→‎Source)

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764


Design Notes

Site specific mutation on plasmid with part BBa_K191003.

Source

Mutagenesis kit of Stratagene was used.

BBa_K191003

References