Device

Part:BBa_K283001

Designed by: Chenli   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 14:19, 21 October 2009 by NelsonChu (Talk | contribs)

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pT7-rbs-(sp_eGFP_streptavidin_AIDAc)-Terminators

This device is for the polar expression of the biotin-binding protein Streptavidin fused with the reporter-green fluorescent protein on the "head" of E.coli strain YBE01.This part does not pose any biological threat.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 2443
    Illegal PstI site found at 1795
    Illegal PstI site found at 2381
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 2443
    Illegal PstI site found at 1795
    Illegal PstI site found at 2381
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 2443
    Illegal BamHI site found at 89
    Illegal BamHI site found at 1021
    Illegal BamHI site found at 2645
    Illegal XhoI site found at 2851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 2443
    Illegal PstI site found at 1795
    Illegal PstI site found at 2381
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 2443
    Illegal PstI site found at 1795
    Illegal PstI site found at 2381
    Illegal AgeI site found at 1063
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
Parameters
None