Coding

Part:BBa_K191008

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-19)
Revision as of 14:08, 21 October 2009 by Nltt1987 (Talk | contribs)

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Promoter LacI - RBS - LovTAP(ILE427PHE) - Term

Mutated version of BBa_K191003 : the amino acid #427 ILE was mutated into PHE.

Usage and Biology

The reasons and choix of this mutation are given by the results of modeling, under [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Results/Mutations Mutations]' section.

The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764


[edit]
Categories
//function/sensor/light
Parameters
input_sIPTG
positive_regulators
protein_outLovTAP